【英文标准名称】:StandardGuideforEvaluatingNonconventionalMicrobiologicalTestsUsedforEnumeratingBacteria
【原文标准名称】:评价细菌计数用非常规微生物试验的标准指南
【标准号】:ASTME1326-2008
【标准状态】:现行
【国别】:美国
【发布日期】:2008
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E35.15
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:
【英文主题词】:Accuracyassessment;Antimicrobialformulations/testing;Bacteria/bacterialcontrol;Heterotrophicplatecountprocedure;Microbiologicalexamination;Nonconventionaltestingprocedures;Reproducibility;Samplingbiologicalmaterials;Selectivity
【摘要】:Thisguideshouldbeusedbyproducersandpotentialproducersofnonconventionalteststodeterminetheaccuracy,selectivity,specificity,andreproducibilityofthetests,asdefinedinPracticesE691andD3870.Resultsofsuchstudiesshouldidentifythelimitationsandindicatetheutilityorapplicabilityofthenonconventionaltest,orboth,foruseondifferenttypesofsamples.Nonconventionaltestusersandpotentialusersshouldemploythisguidetoevaluateresultsofthenonconventionaltestascomparedtotheirpresentmethods.PracticesD5245andD5465shouldbereviewedinregardstotheconventionalmicrobiologicalmethodsemployed.Ifconventionalmethodshavenotbeenusedformonitoringthesystems,thenguidelinesareincludedforobtainingmicrobiologicalexpertise.Utilizationofanonconventionaltestmayreducethetimerequiredtodeterminethemicrobiologicalstatusofthesystemandenableanimprovementintheoveralloperatingefficiency.Inmanycases,thefindingsofasignificantlyhighlevelofbacteriaindicatestheneedforanadditionofanantimicrobialagent.Byaccuratelydeterminingthisinashortertimeperiodthanbyconventionalmethods,treatmentwithantimicrobialagentsmaycircumventmoreseriousproblemsthanifthetreatmentwerepostponeduntilconventionalresultswereavailable.Iftheantimicrobialtreatmentprogramreliesonaninaccuratenonconventionaltest,thenunnecessarylossofproductandproblemsassociatedwithinappropriateselectionorimproperdosingwithantimicrobialagentswouldexist.Sincemanymethodsbasedonentirelydifferentchemicalandmicrobiologicalprinciplesareconsidered,itisnotpossibletoestablishauniquedesignandrecommendaspecificmethodofstatisticalanalysesforthecomparisonstobemade.Itisonlypossibletopresentguidesthatshouldbefollowedwhileperformingtheexperiments.Itisalsorecommendedthatastatisticianbeinvolvedinthestudy.1.1Thepurposeofthisguideistoassistusersandproducersofnonconventionaltestsindeterminingtheapplicabilityofthetestforprocessingdifferenttypesofsamplesandevaluatingtheaccuracyoftheresults.ConventionalproceduressuchastheHeterotrophic(Standard)PlateCount,theMostProbableNumber(MPN)methodandtheSpreadPlateCountarewidelycitedandacceptedfortheenumerationofmicroorganisms.However,thesemethodshavetheirlimitations,suchasperformancetimeanddegreeofaccuracy.Itistheselimitationsthathaverecentlyledtothemarketingofavarietyofnon-conventionalprocedures,testkitsandinstruments.1.2Aconventionaltestisonethatiswidelyacceptedandpublishedasastandardmicrobiologicalmethodorrelatedprocedure.Anew,nonconventionaltestmethodwillattempttoprovidethesameinformationthroughthemeasurementofadifferentparameter.Thisguideisdesignedtoassistinvestigatorsinassessingtheaccuracyandprecisionofnonconventionalmethodsintendedforthedeterminationofmicrobialpopulationdensitiesoractivities.1.3ItisrecognizedthattheHeterotrophicPlateCountdoesnotrecoverallmicroorganismspresentinaproductorasystem(1,2).Whenthisproblemoccursduringthecharacterizationofamicrobiologicalpopulation,alternativestandardenumerationproceduresmaybenecessary,asinthecaseofsulfate-reducingbacteria.Atothertimes,chemicalmethodsthatmeasuretheratesofappearanceofmetabolicderivativesortheutilizationofcontaminatedproductcomponentsmightbeindicated.Inevaluatingnonconventionaltests,theuseofthesealternativestandardproceduresmaybetheonlymeansavailableforestablishingcorrelation.Insuchcases,thisguidecanserveasaref......
【中国标准分类号】:C05
【国际标准分类号】:07_100_10
【页数】:3P.;A4
【正文语种】:英语